Viabilty Test of Fish Scales Collagen Gourami (Oshpronemus Gouramy) on Human Gingival Fibroblasts Cells

Periodontal disease is a pathological inflammatory condition of the periodontal tissues surrounding teeth, including Human Gingival Fibroblasts (HGF) which one major components tissue formation in periodonsium. HGF regeneration with accelerating proliferation engineering therapy needs. Generally, using regenerative materials from cow or pig as therapies, but these have some flaws so this research to find alternative scaffold collagen type 1 derived fresh water fish scales, are gourami scales. This was conducted test viability scales gouramy against for 24 hours. study aimed determine concentration scale can maintain human gingival fibroblast cells taken healthy gingiva and planted 96 well plates. Fish 0.32 mg/ml, 0.16 0.04 0.02 mg/ml 0.01 were added each incubated during MTT Assay performed see cells. The increased after addition on until mg/ml. highest shown has potential engeneering shows HGF.